Two-photon microscope provides unprecedented brain-imaging ability

Dec 5, 2021 Radiology Updates

Two-photon fluorescence microscopy has significantly enhanced researchers’ ability to monitor brain activity with a microscope that provides a resolution high enough to see individual neurons and their neighbors.

The approach leverages the brief, orders-of-magnitude increase in calcium that a neuron experiences when firing. When the laser is pointed at the neuron, and the neuron is firing, calcium comes in, the protein finds the calcium and ultimately, fluoresces.

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