Two-photon fluorescence microscopy has significantly enhanced researchers’ ability to monitor brain activity with a microscope that provides a resolution high enough to see individual neurons and their neighbors.
The approach leverages the brief, orders-of-magnitude increase in calcium that a neuron experiences when firing. When the laser is pointed at the neuron, and the neuron is firing, calcium comes in, the protein finds the calcium and ultimately, fluoresces.
Read more: https://www.sciencedaily.com/releases/2021/12/211202123008.htm
